CRAT Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P43155 |
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Clone Names | 80513058 |
Gene ID | 1384 |
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Other Names | Carnitine O-acetyltransferase, Carnitine acetylase, Carnitine acetyltransferase, CAT, CrAT, CRAT, CAT1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6565b was selected from the C-term region of human CRAT. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | CRAT (HGNC:2342) |
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Synonyms | CAT1 |
Function | Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta- oxidation (PubMed:15099582, PubMed:29395073). Responsible for the synthesis of short- and branched-chain acylcarnitines (PubMed:23485643). Active towards some branched-chain amino acid oxidation pathway (BCAAO) intermediates (PubMed:23485643). Trans-2- enoyl-CoAs and 2-methylacyl-CoAs are poor substrates (PubMed:23485643). |
Cellular Location | Endoplasmic reticulum. Peroxisome. Mitochondrion inner membrane; Peripheral membrane protein; Matrix side [Isoform 2]: Peroxisome |
Tissue Location | Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney |
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Provided below are standard protocols that you may find useful for product applications.
Background
CRAT is carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments.
References
Govindasamy,L., J. Struct. Biol. 146 (3), 416-424 (2004)Jogl,G., Cell 112 (1), 113-122 (2003)
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