DAPK2 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9UIK4 |
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Clone Names | 3010602 |
Gene ID | 23604 |
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Other Names | Death-associated protein kinase 2, DAP kinase 2, DAP-kinase-related protein 1, DRP-1, DAPK2 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7218a was selected from the N-term region of humanPK2 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | DAPK2 |
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Function | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421). |
Cellular Location | Cytoplasm. Cytoplasmic vesicle, autophagosome lumen |
Tissue Location | Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle |
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Provided below are standard protocols that you may find useful for product applications.
Background
DAPK2 belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.
References
Satoh, A., et al., Br. J. Cancer 86(11):1817-1823 (2002).Chan, M.W., et al., Clin. Cancer Res. 8(2):464-470 (2002).Wong, T.S., et al., Clin. Cancer Res. 8(2):433-437 (2002).Shani, G., et al., EMBO J. 20(5):1099-1113 (2001).Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).
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