AlaRS (AARS) Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P49588 |
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Clone Names | 71228101 |
Gene ID | 16 |
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Other Names | Alanine--tRNA ligase, cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, Renal carcinoma antigen NY-REN-42, AARS {ECO:0000255|HAMAP-Rule:MF_03133} |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7558a was selected from the N-term region of human AARS. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | AARS1 |
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Synonyms | AARS {ECO:0000255|HAMAP-Rule:MF_03133} |
Function | Catalyzes the attachment of alanine to tRNA(Ala) in a two- step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (PubMed:27622773, PubMed:27911835, PubMed:28493438, PubMed:33909043). Also edits incorrectly charged tRNA(Ala) via its editing domain (PubMed:27622773, PubMed:27911835, PubMed:28493438). |
Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03133, ECO:0000269|PubMed:27911835} |
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Provided below are standard protocols that you may find useful for product applications.
Background
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
References
Shiba,K.,Biochemistry 34 (33), 10340-10349 (1995)Ripmaster,T.L.,Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4932-4936 (1995)Sang Lee,J.,Cell 127 (3), 635-648 (2006)
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