ILK1 Antibody (T197) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q13418 |
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Other Accession | NP_004508 |
Clone Names | 70903102 |
Gene ID | 3611 |
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Other Names | Integrin-linked protein kinase, 59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7651g was selected from the T197 region of human ILK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | ILK (HGNC:6040) |
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Function | Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling (PubMed:10712922). Focal adhesion protein part of the complex ILK-PINCH (PubMed:10712922). This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway (PubMed:10712922). Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells (PubMed:10712922). Regulates cell motility by forming a complex with PARVB (PubMed:32528174). Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715). |
Cellular Location | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium {ECO:0000250|UniProtKB:O55222}. Cytoplasm, myofibril, sarcomere |
Tissue Location | Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver |
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Provided below are standard protocols that you may find useful for product applications.
Background
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. The ILK protein is a serine/threonine protein kinase with 4 ankyrin-like repeats. ILK regulates integrin-mediated signal transduction.
References
Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003).Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003).Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003).Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003).Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).
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