Caspase-6, human recombinant protein
Caspase-6
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P55212 |
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Calculated MW | large (18 kDa) and small (11 kDa) |
Gene ID | 839 |
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Gene Symbol | CASP6 |
Other Names | Caspase-6, CASP-6, Apoptotic protease Mch-2 |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Caspase-6 |
Application Notes | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
Format | Lyophilized powder |
Storage | -70°C; Lyophilized powder |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-6 (also know as Mch2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-6 consists of large (18 kDa) and small (11 kDa) subunits which associate to form the active enzyme. Active caspase-6 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and henomic maintenance. The recombinant active human caspase-6 was expressed in E. coli. The active caspase-6 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VEID-pNA or Ac-VEID-AFC .
References
Fernandes-Alnemri T.,et al.Cancer Res. 55:2737-2742(1995).
Srinivasula S.M.,et al.J. Biol. Chem. 271:27099-27106(1996).
Bartke T.,et al.Mol. Cell 14:801-811(2004).
Suzuki A.,et al.Oncogene 23:7067-7075(2004).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
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