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Caspase-1, mouse recombinant protein
Caspase 1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P29452 |
|---|---|
| Calculated MW | large (20 kD) and small (10 kD) subunits. |
| Gene ID | 12362 |
|---|---|
| Gene Symbol | CASP1 |
| Other Names | Caspase-1, Short name=CASP-1, Interleukin-1 beta convertase, Short name=IL-1BC, Interleukin-1 beta-converting enzyme, Short name=ICE, IL-1 beta-converting enzyme, p45 |
| Gene Source | Mouse |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Target/Specificity | Caspase-1 |
| Application Notes | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
| Format | Semi-Dry |
| Storage | The lyophilized caspase-1 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active mouse caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA or Ac-YVAD-AFC
References
Nett-Fiordalisi M.A.,et al.J. Immunol. 149:3254-3259(1992).
Molineaux S.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:1809-1813(1993).
Casano F.J.,et al.Genomics 20:474-481(1994).
van de Craen M.,et al.FEBS Lett. 403:61-69(1997).
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