alpha-Fodrin Blocking Peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P16546 |
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Gene ID | 20740 |
Calculated MW | 284597 Da |
Application & Usage | The peptide is used for blocking the antibody activity of α-Fodrin. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C. |
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Other Names | Spectrin alpha chain, non-erythrocytic 1, Alpha-II spectrin, Fodrin alpha chain, Sptan1, Spna2, Spta2 |
Target/Specificity | alpha-Fodrin |
Formulation | 50 µg (0.5 mg/ml) α-Fodrin peptide in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | alpha-Fodrin Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Sptan1 |
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Synonyms | Spna2, Spta2 |
Function | Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. |
Cellular Location | Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. |
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