UbcH1, human recombinant protein (GST-tag)
UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
|Calculated MW||22.4 kDa|
|Other Names||UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme|
|Storage||-80°C; Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT.|
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Provided below are standard protocols that you may find useful for product applications.
Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM.
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Furukawa Y.,et al.Electrophoresis 21:338-346(2000).
Li W.B.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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