Heat Shock Protein 90 (Hsp90), human recombinant protein
Heat Shock Protein 90, HSP90
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P08238 |
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Concentration | 5 |
Calculated MW | 83.1kDa |
Gene ID | 3326 |
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Gene Symbol | HSP90AB1 |
Other Names | Heat Shock Protein 90, HSP90 |
Gene Source | Human |
Source | Sf9 cells |
Assay&Purity | SDS-PAGE; ≥99% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | PEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD |
Format | Liquid |
Storage | -80°C; 5mg/ml in 40 mM HEPES/KOH, pH 7.5, containing 400 mM potassium chloride and 5% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
Hsp90 is a molecular chaperone and is one of the most abundant proteins in unstressed cells. In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90α showing 85% sequence identity to Hsp90β. The recombinant Hsp90 produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90 subunit.
References
Rebbe N.F.,et al.Gene 53:235-245(1987).
Rebbe N.F.,et al.J. Biol. Chem. 264:15006-15011(1989).
Hoffmann T.,et al.Gene 74:491-501(1988).
Lu L.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
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