Epigen (EPGN), human recombinant protein
EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q6UW88 |
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Calculated MW | 7.9 kDa |
Gene ID | 255324 |
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Gene Symbol | EPGN |
Other Names | EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen. |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Results | 150-300 ng/ml |
Application Notes | Reconstitute in sterile dH₂O not less than 100 µg/ml.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Format | Lyophilized protein |
Storage | -20°C; Sterile filtered and lyophilized from 20 mM PBS buffer pH-7.4 containing 130 mM sodium chloride |
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Background
EPGN is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. EPGN is produced in numerous tissues, including the testis, liver, heart and in certain tumor cells. EPGN is mitogenic for fibroblasts and epithelial cells. Human EPGN is originally synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human epigen produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa. Epigen is purified by proprietary chromatographic techniques.
References
Johnstone L.S.,et al.Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
Kochupurakkal B.S.,et al.J. Biol. Chem. 280:8503-8512(2005).
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