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PARP-1, human recombinant protein
Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P09874 |
|---|---|
| Calculated MW | 116.0 kDa |
| Gene ID | 142 |
|---|---|
| Gene Symbol | PARP-1 |
| Other Names | Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP |
| Gene Source | Human |
| Source | Baculovirus |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Application Notes | Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 µL dH₂O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 µL dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH 8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers. |
| Format | Lyophilized protein |
| Storage | -70°C; Lyophilized solid |
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Provided below are standard protocols that you may find useful for product applications.
Background
Poly-ADP-ribose metabolism plays a major role in a wide range of biological processes, such as maintenance of genomic stability, transcriptional regulation, energy metabolism and apoptosis. PARP1 Enzyme, is highly purified and enzymatically active human PARP1, expressed in a baculovirus expression system. Upon addition of NAD+ and cofactors the PARP1 is automodified by the addition of ADP-ribose monomers to form poly(ADP-ribose) polymer. The PARP1 is supplied lyophilized, and upon reconstitution with water, it retains its native poly ADP-ribosylation enzymatic activity. It is useful for high-throughput enzymatic assays, visualization of the automodification reaction by SDS-PAGE and Western blotting, ELISA, standard for SDS-PAGE, and in other assays.
References
Uchida K.,et al.Biochem. Biophys. Res. Commun. 148:617-622(1987).
Kurosaki T.,et al.J. Biol. Chem. 262:15990-15997(1987).
Cherney B.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:8370-8374(1987).
Auer B.,et al.DNA 8:575-580(1989).
Gregory S.G.,et al.Nature 441:315-321(2006).
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