PARP-1, human recombinant protein
Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP
|Calculated MW||116.0 kDa|
|Other Names||Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP|
|Application Notes||Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 µL dH₂O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 µL dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH 8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers.|
|Storage||-70°C; Lyophilized solid|
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Provided below are standard protocols that you may find useful for product applications.
Poly-ADP-ribose metabolism plays a major role in a wide range of biological processes, such as maintenance of genomic stability, transcriptional regulation, energy metabolism and apoptosis. PARP1 Enzyme, is highly purified and enzymatically active human PARP1, expressed in a baculovirus expression system. Upon addition of NAD+ and cofactors the PARP1 is automodified by the addition of ADP-ribose monomers to form poly(ADP-ribose) polymer. The PARP1 is supplied lyophilized, and upon reconstitution with water, it retains its native poly ADP-ribosylation enzymatic activity. It is useful for high-throughput enzymatic assays, visualization of the automodification reaction by SDS-PAGE and Western blotting, ELISA, standard for SDS-PAGE, and in other assays.
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