Human recombinant protein Beta-Enolase
Human Recombinant Beta-Enolase
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P13929 |
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Concentration | 0.5 |
Calculated MW | 49 kDa (454 aa, 1-434 aa + NT His Tag) |
Gene ID | 2027 |
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Gene Symbol | ENO3 |
Other Names | ENO3, MSE |
Gene Source | Human |
Source | E. Coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | >1.5 units/ml |
Sequence | MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK |
Format | Liquid |
Storage | -80°C; 0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, 20% glycerol and 0.1 mM NaCl. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Beta-enolase, also known as ENO3, is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. ENO3 play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Recombinant human ENO3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
References
Peshavaria M.,et al.Nucleic Acids Res. 17:8862-8862(1989).
Cali L.,et al.Nucleic Acids Res. 18:1893-1893(1990).
Peshavaria M.,et al.Biochem. J. 275:427-433(1991).
Giallongo A.,et al.Eur. J. Biochem. 214:367-374(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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