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Bacterial recombinant protein GOR
Bacterial Recombinant GOR
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Concentration | 1 |
|---|---|
| Calculated MW | 51.2 kDa (473 aa, 1-450 aa + NT His-Tag) |
| Other Names | Glutathione oxidoreductase, ECK3485, gorA, JW3467. |
|---|---|
| Gene Source | Bacteria |
| Source | E. Coli |
| Assay&Purity | SDS-PAGE; ≥90% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | >52 unit/ml |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR |
| Format | Liquid |
| Storage | -80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1 M NaCl and 1 mM DTT |
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Provided below are standard protocols that you may find useful for product applications.
Background
Gor, also known as Glutathione oxido-reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of this protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form.
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