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Bacterial recombinant protein SurA
Bacterial Recombinant SurA
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P0ABZ6 |
|---|---|
| Concentration | 1 |
| Calculated MW | 47.3 kDa (429 aa, 21-428 aa + NT His-Tag) |
| Gene ID | 12932020 |
|---|---|
| Gene Symbol | SURA |
| Other Names | PPIase surA, Rotamase surA, Survival protein A, Chaperone surA. |
| Gene Source | Bacteria |
| Source | E. Coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | >450 nmoles/min/mg |
| Sequence | MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN |
| Format | Liquid |
| Storage | -80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E.coli in stationary phase and required for pilus biogenesis.
References
Almiron M.,et al.Unpublished observations (JAN-1993).
Yura T.,et al.Nucleic Acids Res. 20:3305-3308(1992).
Blattner F.R.,et al.Science 277:1453-1462(1997).
Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).
Roa B.B.,et al.J. Bacteriol. 171:4767-4777(1989).
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