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Human recombinant protein AK1
Human Recombinant AK1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P00568 |
|---|---|
| Concentration | 1 |
| Calculated MW | 23.7 kDa (214 aa, 1-194 aa + NT His Tag) |
| Gene ID | 203 |
|---|---|
| Gene Symbol | AK1 |
| Other Names | Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase. |
| Gene Source | Human |
| Source | E. Coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | >6.0 units/ml |
| Sequence | MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK |
| Format | Liquid |
| Storage | -80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
References
von Zabern I.,et al.Eur. J. Biochem. 68:281-290(1976).
Matsuura S.,et al.J. Biol. Chem. 264:10148-10155(1989).
Noma T.,et al.Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
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