Human recombinant protein UBE2H (UbcH2)
Human recombinant UBE2H (UbcH2)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P62256 |
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Calculated MW | 21.649 kDa. (His Tagged) |
Gene ID | 7328 |
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Gene Symbol | UBE2H |
Other Names | Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H, GID3, UBC8, UBCH. |
Gene Source | Human |
Assay&Purity | RP-HPLC; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; 75 µL of a 40 µM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin-conjugating enzyme E2 H (UBE2H) is a member of the ubiquitin-conjugating enzyme family. Protein modification with ubiquitin is a vital cellular apparatus for directing abnormal or short-lived proteins for degradation. Ubiquitination requires at least 3 classes of enzymes: ubiquitin-activating enzymes (E1s) ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2H receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2H protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and Zebrafish homologs.
References
Kaiser P.,et al.J. Biol. Chem. 269:8797-8802(1994).
Lin L.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).
Scherer S.W.,et al.Science 300:767-772(2003).
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