PF-4/CXCL4, murine recombinant protein
Platelet Factor-4, CXCL4, Oncostatin A, Ironplact
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9Z126 |
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Calculated MW | 10.3 kDa |
Gene ID | 56744 |
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Gene Symbol | CXCL4 |
Other Names | Platelet Factor-4, CXCL4, Oncostatin A, Ironplact |
Gene Source | Mouse |
Source | E. Coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES |
Target/Specificity | CXCL4 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Format | Lyophilized powder |
Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the α-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant murine PF-4 is an 8.2 kDa protein containing 76 amino acid residues, including the four highly conserved residues present in CXC chemokines.
References
Watanabe O.,et al.J. Hum. Genet. 44:173-176(1999).
Zhang C.,et al.Blood 98:610-617(2001).
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