Human CellExp CD200/OX-2, human recombinant protein
CD200, MOX1, MOX2, MRC, OX-2, My033
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P41217 |
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Calculated MW | This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 23.4 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 35-45 kDa due to glycosylation. |
Gene ID | 4345 |
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Gene Symbol | CD200 |
Other Names | CD200, MOX1, MOX2, MRC, OX-2, My033 |
Gene Source | Human |
Source | HEK293 cells |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 µg/ml. The concentration of rhCD200 that produces 50% of the optimal binding response is approximately 0.5‑16 ng/ml. |
Target/Specificity | CD200/OX-2 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD200 also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
References
Hou Z.F.,et al.Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
Mao Y.M.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
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