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Human CellExp PD-1 /PDCD1, C-Fc Tag, mouse recombinant protein
PD, PD 1, PD 1 protein, PD 1 recombinant protein, mouse PD 1 protein, mouse recombinant PD 1 protein
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q02242 |
|---|---|
| Calculated MW | This protein is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 43.2 kDa. The predicted N-terminus is Ser 21. DTT-reduced Protein migrates as 60-66 kDa in SDS-PAGE due to glycosylation. |
| Gene ID | 18566 |
|---|---|
| Gene Symbol | PDCD1 |
| Other Names | PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l |
| Gene Source | Mouse |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥92% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-1 /PDCD1 Fc Chimera at 2 µg/mL (100 µl/well) can bind biotinylated Mouse PD-L1 with a linear range of 32 - 500 ng/mL. |
| Target/Specificity | PD-1/PDCD1 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. It can also be used as an immunogen, as a protein standard, or in cell biology research applications.
References
Ishida Y.,et al.EMBO J. 11:3887-3895(1992).
Zhang X.,et al.Immunity 20:337-347(2004).
Lin D.Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:3011-3016(2008).
Lazar-Molnar E.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10483-10488(2008).
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