Ubiquitin-TAMRA-labeled (TMR-Ub) recombinant protein
UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Concentration | ≥ 8 |
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Calculated MW | 9.133 kDa |
Other Names | UBB, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A. |
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Assay&Purity | RP-HPLC; ≥95% |
Assay2&Purity2 | N/A; |
Target/Specificity | Ubiquitin |
Format | Liquid |
Storage | -80°C; ≥ 8 mg/mL in PBS |
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Provided below are standard protocols that you may find useful for product applications.
Background
Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, BioVision’s TAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.
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