Active NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP) recombinant protein
primary alcohol dehydrogenase; ADH; aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-spec
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P35630 |
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Calculated MW | ~40.9 kDa (SDS-PAGE) |
Gene ID | 5759 |
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Gene Symbol | ADH1 |
Other Names | primary alcohol dehydrogenase; ADH; aliphatic alcohol dehydrogenase; ethanol dehydrogenase; NAD-specific aromatic alcohol dehydrogenase; NADP-aldehyde dehydrogenase; yeast alcohol dehydrogenase; alcohol dehydrogenase activity; aldehyde dehydrogenase (NADP) activity |
Gene Source | E. Coli |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -20°C; 1 mg/mL solution in 50 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl and 50% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2-ketones to produce secondary alcohols. Is also active with acetaldehyde and propionaldehyde.
References
Kumar A.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:10188-10192(1992).
Samuelson J.,et al.Arch. Med. Res. 23:31-33(1992).
Shimon L.J.W.,et al.Acta Crystallogr. D 62:541-547(2006).
Goihberg E.,et al.Proteins 72:711-719(2008).
Goihberg E.,et al.Biochemistry 49:1943-1953(2010).
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