Histone H4 (1-103 aa), Human recombinant protein
Histone H4 (1-103 aa), Human recombinant
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P62805 |
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Calculated MW | 11.5 kDa (1-136 aa) |
Gene ID | 8359 |
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Gene Symbol | HIST1H4A |
Other Names | Histone H4, H4 |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Histone H4 |
Application Notes | Resuspend in buffer of choice. |
Format | Lyophilized powder |
Storage | -80°C; Lyophilized powder. Recommended buffer is 50 mM NaPO₄ containing 100 mM sodium chloride and 20% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
H2A is a core component of nucleosome. A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around an octamer of histones. The octamer is composed of two of each histone H2A, H2B, H3, and H4. DNA accessibility is regulated via a complex set of post-translational modifications of these histones, also called histone code, and nucleosome remodeling. Histones H2A and H2B form a dimer. Histones H3 and H4 form a tetramer. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core. Histone H4 undergoes many modifications which include acetylation, phosphorylation and methylation that are important for regulation of gene transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
References
Sierra F.,et al.Nucleic Acids Res. 11:7069-7086(1983).
Pauli U.,et al.Science 236:1308-1311(1987).
Albig W.,et al.Genomics 10:940-948(1991).
Drabent B.,et al.DNA Cell Biol. 14:591-597(1995).
Albig W.,et al.Gene 184:141-148(1997).
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