Active Transglutaminase 2 (His-tagged), human recombinant protein
TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransfer
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P21980 |
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Calculated MW | 79.5 kDa (1-687 aa + N-terminal polyhistidine tag). |
Gene ID | 7052 |
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Gene Symbol | TGM2 |
Other Names | TG2, tTG, tissue transglutaminase, transglutaminase C, TGC, protein-glutamine-gamma-glutamyltransferase, TGM2 |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Results | >400 mU/mg |
Sequence | 1-687 aa of human transglutaminase 2 + N-terminal polyhistidine tag |
Target/Specificity | Transglutaminase 2 |
Application Notes | Reconstitute to 0.1-0.5 mU/ µl in 20 mM Tris, 120 mM NaCl, 10 mM DTT, pH 7.5 containing 20% glycerol. Aliquot and store at –80°C. Avoid repeated freezing and thawing cycles. |
Format | Lyophilized powder |
Storage | -80°C; Lyophilized from 5 mg/ml solution in a proprietary buffer. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Tissue transglutaminase, a 78-kDa calcium dependent enzyme (EC 2.3.2.13), is found both in the intracellular and the extracellular spaces of various types of tissues. TG2 crosslinks proteins between the ε-amino group of a lysine residue and the γ-carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). TG2 also possesses deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Intracellular TG2 is thought to play an important role in apoptosis, while extracellular TG2 has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility.
References
Gentile V.,et al.J. Biol. Chem. 266:478-483(1991).
Fraij B.M.,et al.J. Biol. Chem. 267:22616-22623(1992).
Fraij B.M.,et al.Biochim. Biophys. Acta 1306:63-74(1996).
Bayardo M.P.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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