PKA cg, Active recombinant protein
PKA, cAMP-dependent protein kinase catalytic subunit gamma
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P22612 |
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Concentration | 0.1 µg/ µl |
Calculated MW | 65.0 kDa |
Gene ID | 5568 |
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Gene Symbol | PKA |
Other Names | PKA, cAMP-dependent protein kinase catalytic subunit gamma |
Source | Baculovirus (Sf9 insect cells) |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
PKA C-γ (PKAcg) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-α and C-β and showed tissue-specific expression. Whereas at the amino acid level C-α and C-β showed 93% homology, C-γ showed only about 80% homology to both C-α and C-β (1). The PRKACG gene is intronless, contains remnants of a poly (A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2).
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