GDA, human recombinant protein
Guanine deaminase, CYPIN, GUANASE, NEDASIN
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9Y2T3 |
---|---|
Concentration | 1 |
Calculated MW | 53 kDa (477 aa, 1-454 aa + His Tag) |
Gene ID | 9615 |
---|---|
Gene Symbol | GDA |
Other Names | Guanine deaminase, CYPIN, GUANASE, NEDASIN |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | Specific activity is > 0.9 unit/ml |
Sequence | MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV |
Target/Specificity | GDA |
Format | Liquid |
Storage | -20°C; 1 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1 mM DTT. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
References
Yuan G.,et al.J. Biol. Chem. 274:8175-8180(1999).
Kuwahara H.,et al.J. Biol. Chem. 274:32204-32214(1999).
Park K.H.,et al.Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 6:337-345(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.