T4 DNA Ligase (5 u/ µl) recombinant protein
DNA Ligase
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Concentration | 5 U/ µl |
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Other Names | T4 DNA Ligase |
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Source | E. coli |
Assay&Purity | SDS-PAGE; |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; ENZYME STORAGE BUFFER: 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% glycerol (v/v). |
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Provided below are standard protocols that you may find useful for product applications.
Background
T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor.
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