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LIX/CXCL5

     
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Product Information
Primary Accession P50228
Species Mouse
Sequence Ala41-Gln132
Purity > 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC
Endotoxin Level < 1 EU/ µg of protein by LAL method
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10.0-100.0 ng/ml.
Expression System E. coli
Theoretical Molecular Weight 9.8 kDa
Formulation Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.
Additional Information
Gene ID 20311
Other Names C-X-C motif chemokine 5, Cytokine LIX, Small-inducible cytokine B5, GCP-2(1-78), GCP-2(9-78), Cxcl5, Scyb5
Target Background The mouse homolog of ENA-78 is called LIX. ENA-78/LIX is a CXC chemokine that signals through the CXCR2 receptor. It is expressed in monocytes, platelets, endothelial cells, and mast cells. ENA-78/LIX is a chemoattractant for neutrophils. The three naturally occurring variants of human ENA-78; ENA 5-78, ENA 9-78 and ENA 10-78, contain 74, 70, and 69 amino acid residues, respectively, and possess the same biological activity. ENA-78/LIX contains the four conserved cysteine residues present in CXC chemokines, and also contains the 'ELR' motif common to CXC chemokine that bind to the CXCR1 and CXCR2 receptors.
Protein Information
Name Cxcl5
Synonyms Scyb5
Function May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.
Cellular Location Secreted.
Research Areas
Citations (0)
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Cat# PVGS1108
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