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IL-10
- SPECIFICATION
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- PROTOCOLS
- BACKGROUND
| Primary Accession | P29456 |
|---|---|
| Species | Rat |
| Sequence | Ser19-Asn178 |
| Purity | > 97% as analyzed by SDS-PAGE > 97% as analyzed by HPLC |
| Endotoxin Level | < 0.1 EU/ µg of protein by LAL method |
| Biological Activity | The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0 ng/ml, corresponding to a specific activity of 1.0 × 106 IU/mg. |
| Expression System | E. coli |
| Theoretical Molecular Weight | 18.6 kDa |
| Formulation | Lyophilized from a 0.2 µm filtered solution in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Gene ID | 25325 |
|---|---|
| Other Names | Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il10, Il-10 |
| Target Background | Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine produced by a variety of cell lines including T-cells, macrophages and mast cells. IL-10 is classified as a class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24, and IL-26. IL-10 can inhibit the synthesis of pro-inflammatory cytokines such as IFN-gamma, IL-2, IL-3, TNF and GM-CSF. It also stimulates Th2 responses, but suppresses the antigen-presentation capacity of antigen presenting cells. |
| Name | Il10 |
|---|---|
| Synonyms | Il-10 |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Also interferes with antigen presentation by reducing the expression of MHC- class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity). |
| Cellular Location | Secreted {ECO:0000250|UniProtKB:P22301}. |
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Cat# PVGS1653
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