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>   home   >   Products   >   Cell/Tissues/Lysates   >   AUTODOT™ Visualization Dye   

AUTODOT™ Visualization Dye

Monodansylpentane (MDH) Lipid Droplet Staining Tool

     
  •  - AUTODOT™ Visualization Dye SM1000b
    Localization of guayule Small Rubber Particle Protein (SRPP-GFP) to lipid droplets (LD) in a tobacco cell. Shown are representative epifluorescence micrographs of tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) cells, which serve as a well-characterized system for studying protein localization in plant cells. BY-2 cells have been transiently transformed via biolistic bombardment with plasmid DNA-encoding full-length guayule SRPP12 C-terminally fused to the N-terminus of the Green Fluorescent Protein (SRPP-GFP). Following bombardment, cells have been incubated in linoleic acid, which induces an increase in the number and size of LD in these cells, and then incubated with AUTODOTTM, which is a blue-fluorescing marker dye for LD in living cells. The fluorescence attributable to the AUTODOTTM stained LD is false colorized red. The yellow color in the merged images represents obvious co-localizations between SRPP-GFP and AUTODOTTM -stained LD, most of which have coalesced, due to the ectopic overexpression of the fusion protein. These larger coalesced structures are not observed in the neighboring non-transformed cells wherein LD are usually dispersed throughout the cytosol. Similar coalescence of LD has been observed in BY-2 cells transiently overexpressing Arabidopsis LDAP, as well as in various other cells types in which other LD proteins, such as Perilipin-1 and the Ancient Ubiquitous Protein-1, are ectopically overexpressed. Shown also is the corresponding differential interference contrast image. Bar = 10 μm. Plant Signaling & Behavior 8, e27141; 2013
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  •  - AUTODOT™ Visualization Dye SM1000b
    Image of untreated and autophagy induced mouse cerebral cells analyzed by fluorescence microscopy using an inverted microscope equipped with a filter system (excitation filer: 380-420 nm, barrier filter: 450 nm).
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  • SPECIFICATION
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Product Information
DescriptionAUTODOT™ preferentially segregates into the neutral lipid cores of LDs and emits blue fluorescence, compatible with concurrent use of green and red fluorescent reporters in live-cell imaging. It can be used for visualizing LDs in cell cultures and fixed tissues, making it a versatile marker for LDs in fluorescence microscopy. Major lipid-based pathways such as autophagy, lipolysis, fatty acid oxidation, ketogenesis, and cholesterol synthesis are amenable to tracking by AUTODOT™.
Concentration 0.1M
Target/Specificity AUTODOT™ is a fluorophore that displays selective labeling of lipid droplets (LDs).
Format 0.1M MDH supplied in DMSO
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAUTODOT™ Visualization Dye is for research use only and not for use in diagnostic or therapeutic procedures.
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Background

Lipid droplets (LDs) are dynamic cellular organelles that that store neutral lipids, acting at the interface of lipids and energy metabolism. Abnormal lipid droplet dynamics are associated with the pathophysiology of prevalent metabolic diseases, such as obesity, diabetes, atherosclerosis, fatty liver, and cancer. Research tools such as AUTODOTTM have been essential to emerging research that has identified the biologically relevant protein and lipid compositions of LDs in different cell types and physiological states, and revealed key interactions between LDs and other organelles [e.g. peroxisomes, endosomes, endoplasmic reticulum (ER), plasma membrane and mitochondria].

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$ 250.00
Cat# SM1000b
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