- CITATIONS: 36
|Description||AUTODOT™ preferentially segregates into the neutral lipid cores of LDs and emits blue fluorescence, compatible with concurrent use of green and red fluorescent reporters in live-cell imaging. It can be used for visualizing LDs in cell cultures and fixed tissues, making it a versatile marker for LDs in fluorescence microscopy. Major lipid-based pathways such as autophagy, lipolysis, fatty acid oxidation, ketogenesis, and cholesterol synthesis are amenable to tracking by AUTODOT™.|
|Target/Specificity||AUTODOT™ is a fluorophore that displays selective labeling of lipid droplets (LDs).|
|Format||0.1M MDH supplied in DMSO|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AUTODOT™ Visualization Dye is for research use only and not for use in diagnostic or therapeutic procedures.|
Provided below are standard protocols that you may find useful for product applications.
Lipid droplets (LDs) are dynamic cellular organelles that that store neutral lipids, acting at the interface of lipids and energy metabolism. Abnormal lipid droplet dynamics are associated with the pathophysiology of prevalent metabolic diseases, such as obesity, diabetes, atherosclerosis, fatty liver, and cancer. Research tools such as AUTODOTTM have been essential to emerging research that has identified the biologically relevant protein and lipid compositions of LDs in different cell types and physiological states, and revealed key interactions between LDs and other organelles [e.g. peroxisomes, endosomes, endoplasmic reticulum (ER), plasma membrane and mitochondria].
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