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Bassoon Antibody

     
  • IHC - Bassoon Antibody ASM10461
    Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody (ASM10461). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (ASM10461) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.
  • WB - Bassoon Antibody ASM10461
    Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody (ASM10461). Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (ASM10461) at 1:1000.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9UPA5
Other Accession NP_003449.2
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human Bassoon Polyclonal
Target/Specificity Detects ~420kDa. Multiple isoforms can be detected.
Other Names BSN Antibody, ZNF231 Antibody, Neuronal double zine finger protein Antibody
Immunogen NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-198 was sufficient for detection of Bassoon in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cell Junction | Synapse
Research Areas
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Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors (1).

References

1. Anna Dondzillo et al., (2010) J. Comp Neur, 518(7): 1008–1029.

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Cat# ASM10461
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