CHD3 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant CHD3.
|Application ||WB, IHC|
|Calculated MW||226592 Da|
|Other Names||Chromodomain-helicase-DNA-binding protein 3, CHD-3, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Zinc finger helicase, hZFH, CHD3|
|Target/Specificity||CHD3 (NP_001005273, 1654 a.a. ~ 1741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||CHD3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer. Wang Y, et al. Cell, 2009 Aug 21. PMID 19703393.UV radiation regulates Mi-2 through protein translation and stability. Burd CJ, et al. J Biol Chem, 2008 Dec 12. PMID 18922793.Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin. Sun JM, et al. J Biol Chem, 2007 Nov 9. PMID 17827154.Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity. Sillibourne JE, et al. Mol Biol Cell, 2007 Sep. PMID 17626165.The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene. Pastorcic M, et al. FEBS J, 2007 Mar. PMID 17489097.
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