CHD3 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant CHD3.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
---|---|
Primary Accession | Q12873 |
Other Accession | NM_001005273 |
Reactivity | Human |
Host | mouse |
Clonality | Monoclonal |
Isotype | IgG2a Kappa |
Clone Names | 5.0E+12 |
Calculated MW | 226592 Da |
Gene ID | 1107 |
---|---|
Other Names | Chromodomain-helicase-DNA-binding protein 3, CHD-3, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Zinc finger helicase, hZFH, CHD3 |
Target/Specificity | CHD3 (NP_001005273, 1654 a.a. ~ 1741 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Dilution | WB~~1:500~1000 |
Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
Precautions | CHD3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
References
LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer. Wang Y, et al. Cell, 2009 Aug 21. PMID 19703393.UV radiation regulates Mi-2 through protein translation and stability. Burd CJ, et al. J Biol Chem, 2008 Dec 12. PMID 18922793.Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin. Sun JM, et al. J Biol Chem, 2007 Nov 9. PMID 17827154.Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity. Sillibourne JE, et al. Mol Biol Cell, 2007 Sep. PMID 17626165.The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene. Pastorcic M, et al. FEBS J, 2007 Mar. PMID 17489097.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.