Anti-CD90/Thy1 Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P |
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Primary Accession | P01831 |
Host | Rabbit |
Reactivity | Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Thy-1 membrane glycoprotein(Thy1) detection. Tested with WB, IHC-P in Mouse;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 21838 |
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Other Names | Thy-1 membrane glycoprotein, Thy-1 antigen, CD90, Thy1, Thy-1 |
Calculated MW | 18080 MW KDa |
Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Mouse, By Heat Western blot, 0.1-0.5 µg/ml, Mouse, Rat |
Subcellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
Protein Name | Thy-1 membrane glycoprotein |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | E.coli-derived mouse CD90/Thy1 recombinant protein (Position: Q20-C131). Mouse CD90/Thy1 shares 63.4% and 81.3% amino acid (aa) sequence identity with human and rat CD90/Thy1, respectively. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins. |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | Thy1 |
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Synonyms | Thy-1 |
Function | May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. |
Cellular Location | Cell membrane; Lipid-anchor, GPI-anchor. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD90 (Cluster of Differentiation 90) or Thy-1 is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.
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