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Anti-Cytokeratin Peptide 18 Antibody (Monoclonal, CY-90)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IHC-F |
|---|---|
| Primary Accession | Q5BJY9 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human; rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 294853 |
|---|---|
| Other Names | Keratin, type I cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, Krt18 {ECO:0000250|UniProtKB:P05783} |
| Calculated MW | 47761 MW KDa |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 1-2 µg/ml, Human, rat, By Heat Immunohistochemistry(Frozen Section), 0.5-2 µg/ml, Human, rat, - Western blot, 0.5-1 µg/ml, Human, rat |
| Subcellular Localization | Nucleus matrix . Nucleus, nucleolus . Cytoplasm . |
| Tissue Specificity | Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. . |
| Protein Name | Keratin, type I cytoskeletal 18 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | CY-90 |
| Immunogen | The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the intermediate filament family. |
| Name | Krt18 {ECO:0000250|UniProtKB:P05783} |
|---|---|
| Function | When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin- antithrombin complexes by hepatic cells. |
| Cellular Location | Nucleus matrix. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P05783}. Nucleus, nucleolus {ECO:0000250|UniProtKB:P05783}. Cytoplasm |
| Tissue Location | Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue |
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Provided below are standard protocols that you may find useful for product applications.
Background
Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.
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