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Anti-Collagen IV Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P02462 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Collagen alpha-1(IV) chain(COL4A1) detection. Tested with WB in Human;Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 1282 |
|---|---|
| Other Names | Collagen alpha-1(IV) chain, Arresten, COL4A1 |
| Calculated MW | 160615 MW KDa |
| Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat, Mouse |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Tissue Specificity | Highly expressed in placenta. . |
| Protein Name | Collagen alpha-1(IV) chain |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen IV(1654-1669aa ELRTHVSRCQVCMRRT), identical to the related rat and mouse sequence. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the type IV collagen family. |
| Name | COL4A1 (HGNC:2202) |
|---|---|
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. |
| Cellular Location | Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250|UniProtKB:P02463} |
| Tissue Location | Highly expressed in placenta. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Collagen alpha-1(IV) chain is a protein that in humans is encoded by the COL4A1 gene. Type IV collagen is the major structural component of glomerular basement membranes(GBM), forming a'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in COL4A1 exons 24 and 25 are associated with HANAC(autosomal dominant hereditary angiopathy with nephropathy,aneurysms, and muscle cramps).
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