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Anti-ADFP Picoband Antibody

     
  • WB - Anti-ADFP Picoband Antibody ABO11701
    Western blot analysis of ADFP expression in MCF-7 whole cell lysates (lane 1). ADFP at 48KD was detected using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody (Catalog # ABO11701) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
    detail
  • IHC - Anti-ADFP Picoband Antibody ABO11701
    ADFP was detected in paraffin-embedded sections of mouse testis tissues using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody (Catalog # ABO11701) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-ADFP Picoband Antibody ABO11701
    ADFP was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody (Catalog # ABO11701) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
  • IHC - Anti-ADFP Picoband Antibody ABO11701
    ADFP was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody (Catalog # ABO11701) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q99541
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Perilipin-2(PLIN2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 123
Other Names Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Calculated MW 48075 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Membrane; Peripheral membrane protein.
Tissue Specificity Milk lipid globules.
Protein Name Perilipin-2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen E. coli-derived human ADFP recombinant protein (Position: K226-Q418). Human ADFP shares 88.4% amino acid (aa) sequence identity with mouse ADFP.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name PLIN2
Synonyms ADFP
Function Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Cellular Location Membrane {ECO:0000250|UniProtKB:P43883}; Peripheral membrane protein {ECO:0000250|UniProtKB:P43883}. Lipid droplet
Tissue Location Milk lipid globules..
Research Areas
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Background

Adipose differentiation-related protein, also known as perilipin 2 (PLIN2), ADRP or adipophilin, is a protein which in humans is encoded by the ADFP gene. The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

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$ 280.00
Cat# ABO11701
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