Anti-Claudin 5 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O00501 |
Host | Rabbit |
Reactivity | Human, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Claudin-5(CLDN5) detection. Tested with WB in Human;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 7122 |
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Other Names | Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF, CLDN5, AWAL, TMVCF |
Calculated MW | 23147 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Rat, Human |
Subcellular Localization | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. |
Protein Name | Claudin-5 |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5(191-218aa RPDLSFPVKYSAPRRPTATGDYDKKNYV), different from the related mouse sequence by three amino acids and from the related rat sequence by four amino acids. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | CLDN5 |
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Synonyms | AWAL, TMVCF |
Function | Plays a major role in tight junction-specific obliteration of the intercellular space. |
Cellular Location | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
Claudin-5(CLDN5) also known as BEC1, is a protein that in humans is encoded by the CLDN5 gene. By use of yeast artificial chromosomes, Claudin-5 is localized between polymorphic markers D22S944 and D22S941 on chromosome 22q11, both of which are deleted in more than 80% of VCFS patients. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. What's more, it plays a major role in tight junction-specific obliteration of the intercellular space.
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