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Anti-ARF1 Rabbit Monoclonal Antibody
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- BACKGROUND
Application 
| WB, IHC, FC |
|---|---|
| Primary Accession | P84077 |
| Host | Rabbit |
| Isotype | Rabbit IgG |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-ARF1 Rabbit Monoclonal Antibody . Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
| Gene ID | 375 |
|---|---|
| Other Names | ADP-ribosylation factor 1, 3.6.5.2, ARF1 |
| Calculated MW | 20697 MW KDa |
| Application Details | WB 1:500-1:2000 IHC 1:50-1:200 FC 1:50 |
| Subcellular Localization | Golgi apparatus. Cytoplasm, perinuclear region. Cell junction, synapse, synaptosome. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Membrane ; Lipid- anchor. |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: GFA-1 |
| Immunogen | A synthesized peptide derived from human ARF1 |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Name | ARF1 |
|---|---|
| Function | Small GTPase involved in protein trafficking between different compartments (PubMed:8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed:8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed:8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed:8253837). The GTP- bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity). |
| Cellular Location | Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Synapse, synaptosome {ECO:0000250|UniProtKB:P84079}. Postsynaptic density {ECO:0000250|UniProtKB:P84079}. Note=In the GDP-bound form, associates transiently with the membranes via its myristoylated N-terminus where guanine nucleotide-exchange factor (GEF)-mediated nucleotide exchange occurs (By similarity). Following nucleotide exchange, the GTP-bound form undergoes a conformational change, leading to the exposure of a myristoylated N-terminal amphipathic helix that provides stable membrane anchorage (By similarity). {ECO:0000250|UniProtKB:P84080} |
Research Areas
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
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$ 370.00
Cat# ABO13442
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