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Anti-DDAH1 Monoclonal Antibody

     
  •  - Anti-DDAH1 Monoclonal Antibody ABO14604
    Figure 1. Western blot analysis of DDAH1 using anti-DDAH1 antibody (M03136).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human 293T whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: monkey COS-7 whole cell lysates,
    Lane 4: human SH-SY5Y whole cell lysates.
    After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified monoclonal antibody (Catalog # M03136) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH1 at approximately 37 kDa. The expected band size for DDAH1 is at 31 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, ICC, IP, FC
Primary Accession O94760
Host Rabbit
Isotype Rabbit IgG
Reactivity Rat, Human, Mouse, Monkey
Clonality Monoclonal
Format Liquid
Description Anti-DDAH1 Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Additional Information
Gene ID 23576
Other Names N(G), N(G)-dimethylarginine dimethylaminohydrolase 1, DDAH-1, Dimethylarginine dimethylaminohydrolase 1, 3.5.3.18, DDAHI, Dimethylargininase-1, DDAH1 (HGNC:2715), DDAH
Calculated MW 31122 Da
Application Details WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:30
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: AEAG-4
Immunogen A synthesized peptide derived from human DDAH1 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name DDAH1 (HGNC:2715)
Synonyms DDAH
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)- monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue Location Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Research Areas
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$ 370.00
Cat# ABO14604
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