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Anti-Xanthine Oxidase Rabbit Monoclonal Antibody

     
  •  - Anti-Xanthine Oxidase Rabbit Monoclonal Antibody ABO15402
    Western blot analysis of Xanthine Oxidase expression in 293T cell lysate.
    detail
  •  - Anti-Xanthine Oxidase Rabbit Monoclonal Antibody ABO15402
    Figure 2. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
    Xanthine Oxidase was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-Xanthine Oxidase Rabbit Monoclonal Antibody ABO15402
    Figure 3. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
    Xanthine Oxidase was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-Xanthine Oxidase Rabbit Monoclonal Antibody ABO15402
    Figure 4. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
    Xanthine Oxidase was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
  •  - Anti-Xanthine Oxidase Rabbit Monoclonal Antibody ABO15402
    Figure 5. IHC analysis of Xanthine Oxidase using anti-Xanthine Oxidase antibody (M01884-2).
    Xanthine Oxidase was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Xanthine Oxidase Antibody (M01884-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P47989
Host Rabbit
Isotype IgG
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Format Liquid
Description Anti-Xanthine Oxidase Rabbit Monoclonal Antibody . Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Additional Information
Gene ID 7498
Other Names Xanthine dehydrogenase/oxidase, Xanthine dehydrogenase, XD, 1.17.1.4, Xanthine oxidase, XO, 1.17.3.2, Xanthine oxidoreductase, XOR, XDH, XDHA
Calculated MW 146 kDa
Application Details WB 1:500-1:2000
IHC 1:50-1:200
Contents Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names Clone: 19X78
Immunogen A synthesized peptide derived from human Xanthine Oxidase
Purification Affinity-chromatography
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Protein Information
Name XDH
Synonyms XDHA
Function Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
Cellular Location Cytoplasm. Peroxisome. Secreted
Tissue Location Detected in milk (at protein level). {ECO:0000269|Ref.12}
Research Areas
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$ 370.00
Cat# ABO15402
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