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NCF1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, ICC |
|---|---|
| Primary Accession | P14598 |
| Reactivity | Human, Mouse, Monkey |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44682 Da |
| Gene ID | 653361 |
|---|---|
| Positive Control | WB: Jurkat, THP1 cell lysate; IHC: human tonsil tissue; IFC: Jurkat cells |
| Application & Usage | WB; 1:500 – 1:2000, IHC; 1:50 – 1:200, IF/IC; 1:50 – 1:100 |
| Alias Symbol | NCF1 |
| Other Names | NOXO2, SH3PXD1A, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47 phox, Neutrophil cytosol factor 1 |
| Appearance | Colorless liquid |
| Formulation | In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | NCF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NCF1 (HGNC:7660) |
|---|---|
| Synonyms | NOXO2, SH3PXD1A |
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:2547247, PubMed:2550933, PubMed:38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed:38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed:38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed:12732142, PubMed:19801500). |
| Cellular Location | Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side |
| Tissue Location | Detected in peripheral blood monocytes and neutrophils (at protein level). |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production)
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