Anti-EEF2 Antibody (4B3-G7-H5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF |
---|---|
Primary Accession | P13639 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | Mouse IgG2b |
Clone Names | 4B3-G7-H5 |
Calculated MW | 95338 Da |
Gene ID | 1938 |
---|---|
Application & Usage | WB: HL-60, Jurkat, SHSY-5Y, U20S and Hela cell lysates; IF: HeLa cells |
Other Names | EEF2, Eef2, EF-2, EF2, EF2_HUMAN, Elongation factor 2, Eukaryotic translation elongation factor 2, Polypeptidyl tRNA translocase, SCA26 |
Target/Specificity | EEF2 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | In buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Anti-EEF2 Antibody (4B3-G7-H5) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | EEF2 |
---|---|
Synonyms | EF2 |
Function | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl- tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721). |
Cellular Location | Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C- terminal cleavage product. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
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