|Application ||WB, IHC|
|Calculated MW||175491 Da|
|Other Names||Eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220, EIF4G1, EIF4F, EIF4G, EIF4GI|
|Target/Specificity||The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site.|
|Format||affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-EIF4G-S1232 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EIF4F, EIF4G, EIF4GI|
|Function||Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. As a member of the eIF4F complex, required for endoplasmic reticulum stress- induced ATF4 mRNA translation (PubMed:29062139).|
|Cellular Location||Cytoplasm, Stress granule|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Over-expression of eukaryotic translation initiation factor 4 gamma 1 correlates with tumor progression and poor prognosis in nasopharyngeal carcinoma. Tu L, et al. Mol Cancer, 2010 Apr 16. PMID 20398343.
Nuclear assortment of eIF4E coincides with shut-off of host protein synthesis upon poliovirus infection. Sukarieh R, et al. J Gen Virol, 2010 May. PMID 20053821.
NAD(P)H quinone-oxydoreductase 1 protects eukaryotic translation initiation factor 4GI from degradation by the proteasome. Alard A, et al. Mol Cell Biol, 2010 Feb. PMID 20028737.
HIV- 1 protease inhibits Cap- and poly(A)-dependent translation upon eIF4GI and PABP cleavage. Castell贸 A, et al. PLoS One, 2009 Nov 24. PMID 19956697.
The Hsp90 inhibitor geldanamycin abrogates colocalization of eIF4E and eIF4E-transporter into stress granules and association of eIF4E with eIF4G. Suzuki Y, et al. J Biol Chem, 2009 Dec 18. PMID 19850929.
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