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Goat Anti-NCF1 / p47phox Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P14598 |
| Other Accession | NP_000256, 653361, 17969 (mouse), 114553 (rat) |
| Reactivity | Human |
| Predicted | Mouse, Rat, Rabbit, Pig |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 44682 Da |
| Gene ID | 653361 |
|---|---|
| Other Names | Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-NCF1 / p47phox Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NCF1 (HGNC:7660) |
|---|---|
| Synonyms | NOXO2, SH3PXD1A |
| Function | Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:2547247, PubMed:2550933, PubMed:38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed:38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed:38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed:12732142, PubMed:19801500). |
| Cellular Location | Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side |
| Tissue Location | Detected in peripheral blood monocytes and neutrophils (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
References
Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update). Roos D, et al. Blood Cells Mol Dis, 2010 Apr 15. PMID 20167518.
Integrative predictive model of coronary artery calcification in atherosclerosis. McGeachie M, et al. Circulation, 2009 Dec 15. PMID 19948975.
NOX activity is increased in mild cognitive impairment. Bruce-Keller AJ, et al. Antioxid Redox Signal, 2010 Jun 15. PMID 19929442.
Dynamin 2 and c-Abl are novel regulators of hyperoxia-mediated NADPH oxidase activation and reactive oxygen species production in caveolin-enriched microdomains of the endothelium. Singleton PA, et al. J Biol Chem, 2009 Dec 11. PMID 19833721.
Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening. Voss M, et al. BMC Immunol, 2009 Oct 6. PMID 19807924.
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