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Goat anti-Connective Tissue Growth Factor Antibody

Peptide-affinity purified goat antibody

     
  • IF - Goat anti-Connective Tissue Growth Factor Antibody AF4496a
    EB06683 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
    detail
  • IHC - Goat anti-Connective Tissue Growth Factor Antibody AF4496a
    EB06683 (6µg/ml) staining of paraffin embedded Rat Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
    detail
  • IHC - Goat anti-Connective Tissue Growth Factor Antibody AF4496a
    EB06683 Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, Pep-ELISA
Primary Accession P29279
Other Accession NP_001892
Reactivity Human, Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Clone Names CCN2
Calculated MW 38091 Da
Additional Information
Gene ID 1490
Other Names CTGF; CCN2; NOV2; IGFBP8; connective tissue growth factor
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat anti-Connective Tissue Growth Factor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCN2 (HGNC:2500)
Function Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor- induced DNA synthesis.
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000250|UniProtKB:P29268}. Secreted {ECO:0000250|UniProtKB:P29268}
Tissue Location Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Research Areas
Citations (0)
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$ 341.00
Cat# AF4496a
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