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KD-Validated Anti-Kinesin family member 11 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Kinesin family member 11 Rabbit Monoclonal Antibody AGI1001
    Western blotting analysis using anti-Kinesin family member 11 antibody (Cat#60146). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Kinesin family member 11 antibody (Cat#60146, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Kinesin family member 11 Rabbit Monoclonal Antibody AGI1001
    Western blotting analysis using anti-Kinesin family member 11 antibody (Cat#60146). Kinesin family member 11 expression in wild type (WT) and kinesin family member 11 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Kinesin family member 11 antibody (Cat#60146, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Kinesin family member 11 Rabbit Monoclonal Antibody AGI1001
    Flow cytometric analysis of Kinesin family member 11 expression in HAP-1 cells using Kinesin family member 11 antibody (Cat#60146, 1:2,000). Green, isotype control; red, Kinesin family member 11.
    detail
  • ICC - KD-Validated Anti-Kinesin family member 11 Rabbit Monoclonal Antibody AGI1001
    Immunocytochemical staining of HAP-1 cells with Kinesin family member 11 antibody (Cat#60146, 1:1,000). Nuclei were stained blue with DAPI; Kinesin family member 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P52732
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2025
Calculated MW Predicted, 119 kDa, observed, 119 kDa
Gene Name KIF11
Aliases KIF11; Kinesin Family Member 11; TRIP5; KNSL1; HKSP; EG5; Thyroid Receptor-Interacting Protein 5; Kinesin-Like Spindle Protein HKSP; Kinesin-Related Motor Protein Eg5; Kinesin-Like Protein KIF11; TR-Interacting Protein 5; Kinesin-Like Protein 1; TRIP-5; Kinesin-Like 1; MCLMR; Eg5
Immunogen A synthesized peptide derived from human Eg5
Additional Information
Gene ID 3832
Other Names Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5
Protein Information
Name KIF11
Synonyms EG5, KNSL1, TRIP5
Function Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed:19001501, PubMed:37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, spindle pole
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1001
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