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KD-Validated Anti-Histone H1.0 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Histone H1.0 Rabbit Monoclonal Antibody AGI1012
    Western blotting analysis using anti-Histone H1.0 antibody (Cat#AGI1012). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Histone H1.0 antibody (Cat#AGI1012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Histone H1.0 Rabbit Monoclonal Antibody AGI1012
    Western blotting analysis using anti-histone H1.0 antibody (Cat#AGI1012). Histone H1.0 expression in wild-type (WT) and H1-0 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-histone H1.0 antibody (Cat#AGI1012, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-Histone H1.0 Rabbit Monoclonal Antibody AGI1012
    Immunocytochemical staining of C2C12 cells with Histone H1.0 antibody (Cat#AGI1012, 1:1,000). Nuclei were stained blue with DAPI; Histone H1.0 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P07305
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 1965
Calculated MW Predicted, 21 kDa , observed, 28 kDa
Gene Name H1-0
Aliases H1-0; H1.0 Linker Histone; H1.0; H1F0; H1FV; H1 Histone Family Member 0; H1.0, H1(0), H1-0; Histone H1(0); Histone H1.0; Histone H1'; H1 Histone Family, Member 0; H10
Immunogen A synthesized peptide derived from human Histone H1.0
Additional Information
Gene ID 3005
Other Names Histone H1.0, Histone H1', Histone H1(0), Histone H1.0, N-terminally processed, H1-0 (HGNC:4714)
Protein Information
Name H1-0 (HGNC:4714)
Function Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00837, ECO:0000269|PubMed:18993075}. Chromosome {ECO:0000255|PROSITE- ProRule:PRU00837, ECO:0000269|PubMed:18993075}. Note=The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1012
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