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Anti-Histone H1.0 Antibody

Rabbit polyclonal antibody to Histone H1.0

     
  • WB - Anti-Histone H1.0 Antibody AP60009
    Western blot analysis of Histone H1.0 expression in mouse liver (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates.
    detail
  • IH - Anti-Histone H1.0 Antibody AP60009
    Immunohistochemical analysis of Histone H1.0 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P07305
Other Accession P10922
Reactivity Human, Mouse, Rat, Monkey
Host Rabbit
Clonality Polyclonal
Calculated MW 20863 Da
Additional Information
Gene ID 3005
Other Names H1FV; Histone H1.0; Histone H1'; Histone H1(0)
Target/Specificity Recognizes endogenous levels of Histone H1.0 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
IH~~WB (1/500 - 1/1000), IH (1/100 - 1/200)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name H1-0 (HGNC:4714)
Function Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00837, ECO:0000269|PubMed:18993075}. Chromosome {ECO:0000255|PROSITE- ProRule:PRU00837, ECO:0000269|PubMed:18993075}. Note=The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes
Citations (0)
citation

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Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histone H1.0. The exact sequence is proprietary.

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$ 350.00
$ 175.00
Cat# AP60009
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