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KD-Validated Anti-Hsp22 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Hsp22 Rabbit Monoclonal Antibody AGI1014
    Western blotting analysis using anti-Hsp22 antibody (Cat#AGI1014). Total cell lysates (50 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp22 antibody (Cat#AGI1014, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Hsp22 Rabbit Monoclonal Antibody AGI1014
    Western blotting analysis using anti-Hsp22 antibody (Cat#AGI1014). Hsp22 expression in wild type (WT) and Hsp22 shRNA knockdown (KD) 293T cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Hsp22 antibody (Cat#AGI1014, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Hsp22 Rabbit Monoclonal Antibody AGI1014
    Flow cytometric analysis of Hsp22 expression in HepG2 cells using Hsp22 antibody (Cat#AGI1014, 1:2,000). Green, isotype control; red, Hsp22.
    detail
  • ICC - KD-Validated Anti-Hsp22 Rabbit Monoclonal Antibody AGI1014
    Immunocytochemical staining of HepG2 cells with Hsp22 antibody (Cat#AGI1014, 1:1,000). Nuclei were stained blue with DAPI; Hsp22 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9UJY1
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1980
Calculated MW Predicted, 22 kDa , observed, 22 kDa
Gene Name HSPB8
Aliases HSPB8; Heat Shock Protein Family B (Small) Member 8; E2IG1; HSP22; CMT2L; H11; Small Stress Protein-Like Protein HSP22; Heat Shock 27kDa Protein 8; Heat Shock 22kDa Protein 8; Heat Shock Protein Beta-8; E2-Induced Gene 1 Protein; Alpha-Crystallin C Chain; Protein Kinase H11; HSPB8-N1; HSPB8-N2; DHMN2; HMN2A; CRYAC; HspB8; HMN2
Immunogen A synthesized peptide derived from human HSPB8/HSP22
Additional Information
Gene ID 26353
Other Names Heat shock protein beta-8, HspB8, Alpha-crystallin C chain, E2-induced gene 1 protein, Heat shock protein family B member 8, Protein kinase H11, Small stress protein-like protein HSP22, HSPB8, CRYAC, E2IG1, HSP22
Protein Information
Name HSPB8
Synonyms CRYAC, E2IG1, HSP22
Function Involved in the chaperone-assisted selective autophagy (CASA), a crucial process for protein quality control, particularly in mechanical strained cells and tissues such as muscle. Displays temperature-dependent chaperone activity.
Cellular Location Cytoplasm. Nucleus Note=Translocates to nuclear foci during heat shock
Tissue Location Predominantly expressed in skeletal muscle and heart.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1014
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