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KD-Validated Anti-C4BPB Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-C4BPB Rabbit Monoclonal Antibody AGI1018
    Western blotting analysis using anti-C4BPB antibody (Cat#AGI1018). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-C4BPB antibody (Cat#AGI1018, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-C4BPB Rabbit Monoclonal Antibody AGI1018
    Western blotting analysis using anti-C4BPB antibody (Cat#AGI1018). C4BPB expression in wild-type (WT) and C4BPB shRNA knockdown (KD) HepG2 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-C4BPB antibody (Cat#AGI1018, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • ICC - KD-Validated Anti-C4BPB Rabbit Monoclonal Antibody AGI1018
    Immunocytochemical staining of HepG2 cells with C4BPB antibody (Cat#AGI1018, 1:1,000). Nuclei were stained blue with DAPI; C4BPB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P20851
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB5940
Calculated MW Predicted, 28 kDa, observed, 42 kDa
Gene Name C4BPB
Aliases C4BPB; Complement Component 4 Binding Protein Beta; C4b-Binding Protein Beta Chain; C4BP; Complement Component 4 Binding Protein, Beta Chain; Complement Component 4-Binding Protein, Beta; C4b Binding Protein, Beta Chain
Immunogen A synthesized peptide derived from human C4 binding protein
Additional Information
Gene ID 725
Other Names C4b-binding protein beta chain, C4BPB
Protein Information
Name C4BPB
Function Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
Cellular Location Secreted.
Research Areas
Citations (0)
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$ 399.20
$ 149.00
Cat# AGI1018
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